Journal: Frontiers in Cell and Developmental Biology
Article Title: Melatonin Attenuates Chromium (VI)-Induced Spermatogonial Stem Cell/Progenitor Mitophagy by Restoration of METTL3-Mediated RNA N 6 -Methyladenosine Modification
doi: 10.3389/fcell.2021.684398
Figure Lengend Snippet: Melatonin alleviated Cr (VI)-induced damage to male reproductive system and autophagy in mouse testes. (A) H&E staining of testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 200 μm. (B–D) The ratios of only 1–3 layers of germ cell (B) , empty (C) , and abnormal (D) seminiferous tubules in testes from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. (E) The average body weight in different mouse groups. (F) The average testicular index (testis weight/body weight) in different mouse groups. (G) The average sperm number (10 6 /ml) in different mouse groups. (H) The ratio of spermatozoa with progressive motility in different mouse groups. (I) The upper panel: the ratio of abnormal spermatozoa in different mouse groups; The lower panel: images of spermatozoa from different groups of mice. Bar = 50 μm. (J) Immunofluorescence staining for LIN28 in testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 200 μm. (K) The numbers of LIN28 + cells per seminiferous tubule in testes from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. (L) Co-staining analysis for LC3 and LIN28 in testis sections from the control, Cr (VI), melatonin + Cr (VI), and melatonin-treated mice. Bar = 100 μm. Data are presented as the mean ± SEM from five mice, and 300 seminiferous tubules from five mice were analyzed in each group. * p < 0.05; ** p < 0.01.
Article Snippet: A minimum of 300 spermatozoa were observed from at least five randomly selected fields with 20 μm CELL-VU® DRM-600 sperm count slides (Millennium Sciences, United States) and a microscopic stage warmer (KITAZATO, Japan).
Techniques: Staining, Control, Immunofluorescence